FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15042305
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC416460.276859164868682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT77500.051.3967558
TCGACGC17050.048.172287
CCGACGC48050.045.055417
GACGCTC89450.043.943849
TGCGACG18750.041.7790076
CTAGGCG108700.040.5316127
GTCGACG9900.039.8032346
TAGGCGG110950.039.7524458
GCGACGC32150.038.689747
GCCGACG24450.037.0877086
CTCGACG11750.035.5566026
GCTAGGC129700.034.33516
GGCTAGG142300.032.2001955
TCCGACG13500.030.5957376
TGGCTAG155500.029.7154334
GTGCGAC21850.029.1198715
GCGACAT23750.028.9894875
TGCGACA23350.028.8802594
TCCGATC68100.028.09369516-17
AGGCGGG163600.027.0736249
Produced by FastQC (version 0.11.5)