FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15042305
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN467550.31082337447618563No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183630.12207570581769217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC30250.035.0294279
CCCCGTA22050.032.083132
TTCCGAC33650.031.9113318
CCCCGAA35450.031.3399752
GTCCCGT20550.031.2648451
CCGATCG40450.030.11246586-87
CCCCCGT30750.030.0255471
GTTCCGT17300.028.8853421
CGATCGG40350.028.83367586-87
CCGTTAT15900.028.3867764
CCGTTAA17550.027.8836564
CCCGTTA20850.027.8001863
GATCGGC45400.027.35226488-89
ATAGCGC43250.027.00967694-95
GACCCGT10950.026.512461
CCCGTTT47400.026.261343
GCCCCGT28600.026.1257021
ACCCGAT47100.026.01323984-85
CCCGCGT10850.025.8795411
CGGGGAT81500.025.593438
Produced by FastQC (version 0.11.5)