FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004916377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126751
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146600.4688572898833326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72430.2316462039989753No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAAAAAAAAAAAAAAAAAAAAAAA72310.23126241904136272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70240.22464212852254622No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA47120.15069956002252818No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG40590.12981526191244522Clontech Universal Primer Mix Long (96% over 25bp)
TCATAAAAAGGTCAATCCAACCACCCAAAAAAAAAAAAAAAAAAAAAAAA38120.12191568820158688No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGG35200.11257692089968148No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC34140.10918682044077062No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33770.10800348348813193No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGACGTTTGAGATTTGAGAG31670.1012872467299123No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA31480.10067958721369243No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)