FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15674910
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC1347170.8594435310952343No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT1017240.6489606638889793No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1014320.6470978142777215No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG797000.5084558699220602No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA784800.5006727311352984No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG780820.49813364159666623No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC729480.4653806624727032No Hit
CCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGA702800.4483598310931291No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC656230.4186499316423507No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT604260.3854950363351369No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT578700.3691887226146753No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT564850.36035294620511377No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG562380.3587771795818923No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG517650.3302411305710846No Hit
CACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTT466350.297513669934947No Hit
CCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCAT450550.28743386724389486No Hit
GGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGG441890.28190911462968526No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG390790.2493092464326749No Hit
CCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACT382990.24433314130671244No Hit
GCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGG350380.2235291941070156No Hit
GGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGAT343960.21943347681103115No Hit
AGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGAT320460.20444136521358017No Hit
GCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG315420.20122603574757367No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC311250.1985657333917707No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA306990.19584801443836042No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG296340.18905371705483476No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC295840.18873473595701665No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC292180.18639979432098813No Hit
AGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG288000.18373311234322875No Hit
CAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGG286700.1829037614889017No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG279780.1784890630950991No Hit
CGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCG277220.1768558798742704No Hit
CCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGC261710.16696108621995276No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG249070.15889724406711106No Hit
CCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGG248160.15831669846908214No Hit
CGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGC243950.15563087762545366No Hit
CCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATT237640.15160533617098917No Hit
CCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGT234650.14969782920603691No Hit
GACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGC233470.14894503381518617No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA232070.1480518867412955No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG230750.1472097766430557No Hit
CGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTAT220170.14046013661322457No Hit
CCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAAT219350.1399370076128029No Hit
AGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGA218130.1391586937341267No Hit
CCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCT217370.13867384246544318No Hit
CAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG212910.13582853107290568No Hit
AGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGACCG208830.13322564531470996No Hit
CCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCATTTAAAGTT205410.1310438146056341No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC205090.13083966670303052No Hit
CGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCAATCGGTAGTAGCG196710.12549354350359906No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC193330.12333723128234866No Hit
CATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGC192970.12310756489191962No Hit
CCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATA190710.12166577032978179No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG189670.12100228964632015No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA185800.11853337594920801No Hit
CCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCG181600.11585393472753591No Hit
GCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCT180820.11535632421493967No Hit
CGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTC176730.11274705883478756No Hit
GGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGC175720.112102717017195No Hit
TGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCG171310.10928930373443932No Hit
CCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAAT167980.10716488962297072No Hit
CCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAG164510.10495116080411306No Hit
ATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTG162420.1036178198152334No Hit
CACACCCCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACACGCGCG162240.10350298662001886No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGG157730.10062577711769954No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCC41750.048.2394031
CCCGCCA111050.045.5964661
TGCGACA16750.044.5109144
ACCGCCA84300.041.977951
TAGCCGC12200.041.659731
ACCTACG126700.040.2916372
CCTACGG128850.040.061593
TACGGAA129050.039.773765
CACGAAT212750.038.6774645
GCGACAT19900.038.1764835
TCACGAA214050.038.1144454
ATCACGA214700.038.065533
CGGAAAC136800.038.036167
CATCACG215150.037.91972
ATACCGC11050.036.9682351
ACGGAAA139600.036.8991626
ACGACGC14200.036.126671
AACCGCC16700.036.122661
CACCTAC144100.034.973891
TACCGAC13750.034.890821
Produced by FastQC (version 0.11.5)