FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13636683
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC39000.049.6597989
TTCCGAC41350.047.0363358
TTTCCGA53450.037.0770237
GTTTCCG48050.035.5635226
TCCGTTT55050.031.642413
CTCCGTT42750.031.4223822
CGTTTCC54550.030.7314975
GCTCCGT38400.030.4354131
CCGTTTC56450.030.3742684
TCACCCC134550.030.3571329
ATAGCGC64000.029.30298694-95
CCGATCG68750.029.21293486-87
GTCCCGT16400.028.1660121
TTCACCC146000.027.5239548
GTTCACC149150.027.1598037
CCCGTTT40950.027.069383
ATTGCGC18500.026.8138779
CATAGCG74400.026.72170394-95
ACCCGAT77700.026.65667284-85
CGGCATA72250.026.14737990-91
Produced by FastQC (version 0.11.5)