FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330771
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT120240.5158807965261281No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108390.4650392509603045No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA66630.2858710701308709No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66400.2848842722000574No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT65860.28256744227553887No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAG58070.24914502540146588No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATAT55690.2389338120304397No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA44430.19062361767844202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35410.15192397708741012No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT32190.1381088060560218No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC29690.12738274159065818No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28930.12412201799318766No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT28110.12060386884854839No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC28080.12047515607496403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27930.11983159220704222No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC26110.11202301727625752No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT25620.10992070864104624No Hit
TTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTA24800.10640255949640699No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG24470.10498671898697899No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT23630.10138276132661682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC554.2344054E-665.452618
GTCTCGC1850.058.620621
CGGGACA1800.055.9959344
GTATCAA35750.055.816671
ACGGGAC3900.055.3805963
CAACGCA34100.054.4722865
TCAACGC34850.053.5054474
ATCAACG35450.052.599853
AACGCAG36150.051.3832666
CGCAGAG37500.049.534538
GTACTTT68900.049.213531
ACGCAGA39250.047.324977
TCTCGCT2350.045.9541132
GGTATCA17300.045.134491
GGGGTTA41300.044.8015826
ATAGGGG40550.044.386423
ACCTTAG24600.044.084611
CTCGCTC2150.043.5317273
GGGTTAG43050.043.4820987
TAGGGGT42450.042.5693474
Produced by FastQC (version 0.11.5)