FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449622
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATG45460.3135989933927603No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC44970.3102188018669695No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT40970.28262540165643185No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG35270.24330480635641566No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT33450.23074980926062102No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT28130.19405058698060598No Hit
TCATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATG24870.1715619658090178No Hit
TCATAAAAAGGTCAATCCAACCACCCAAATCAAGCGTTACGTACTGCAGT23920.1650085332590151No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT21290.14686587262058662No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC20370.14051939057216295No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG19520.1346557930274237No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC19450.13417290852373928No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT18420.12706760796952585No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG17520.12085909292215487No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA17440.12030722491794413No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC17410.12010027441636509No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCCCGTACTCTGCGTT17040.11754788489689036No Hit
CGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTG16440.1134088748653097No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT15900.10968376583688713No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG15050.10382016829214788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14980.10333728378846348No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGAG24800.089.891976
AAAGGTC16000.087.315067
AATACAG28100.084.201164
ATACAGA27150.082.5491945
ACAGAGA26850.082.364487
AGGTCAA17350.078.465249
CAGAGAA27850.078.3397458
TAACAAG10900.076.355946
AAGGTCA18150.075.9893348
TGTAACA7250.072.158254
TAAAAAG20550.071.743274
AACAAGC12300.066.698387
AGAGAAA32500.066.399469
GAAATAC46800.062.8782772
AAATACA38750.061.5196653
TGAAATA49850.059.287431
TCATGTA9350.057.3557551
CATAAAA36000.056.6412622
TCATAAA36250.054.5727961
CAAGCAA9850.050.6972439
Produced by FastQC (version 0.11.5)