FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12399115
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC36900.048.997629
TTCCGAC37400.048.4462938
TTTCCGA48450.037.829487
GTTTCCG42700.036.1784746
GTTCCGT14400.034.6204031
ATTGCGC16850.034.238319
CGTTTCC49300.031.334365
CCGTTTC53350.030.035644
TCCGTTT54850.029.880983
CCGATCG62500.029.39740286-87
CTCCGTT39300.029.0464532
ATAGCGC60950.029.00403294-95
GCTCCGT35000.028.9471281
TCACCCC130300.028.0677859
CATTGCG21350.027.4711118
CCCCGTT25500.026.90052
CATAGCG70750.026.65018794-95
GTCCCGT14950.026.5339681
CCCGTTT37200.026.463183
TTCACCC137500.026.3928728
Produced by FastQC (version 0.11.5)