FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173702
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57680.4914364975095893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38090.3245287134212943No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA25150.21427926339053693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.20524801014226782No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC22000.187441105152756No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC20830.17747264637872306No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC17200.14654486402851832No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT16460.14024002685519835No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT16350.13930282132943456No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA15940.13580960073340592No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA15870.1352131972170108No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG15370.1309531720999027No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG15200.12950476356008594No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC12620.10752303395580821No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA12430.10590422441130715No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT12170.10368901135041092No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA12040.10258140481996282No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC11990.10215540230825201No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC11770.10028099125672446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39400.072.294831
GTATCAA73850.058.25891
GTGCACG350.00774969251.07741
TATCAAC95350.045.355512
ATCAACG100000.042.949053
CAACGCA102500.042.0756155
AACGCAG106200.040.833766
TCAACGC105750.040.7825134
ACGCAGA124150.034.7382287
CGCAGAG125650.034.3235248
GCAGAGT128950.033.445149
ACTTCGC902.3962797E-433.04798
ATGGGTG7100.029.3241925
GTACTGG12000.029.2985651
ACGCAAG1055.913733E-428.326778
CATGGGT18750.028.2361244
CAGAGTA129650.027.6210610-11
GACCGTT4350.027.3499837
ACGGTAC3500.027.19373
GTATTAG5500.027.0864981
Produced by FastQC (version 0.11.5)