FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173702
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA23830.20303279708137162No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC21380.18215867400754193No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC20610.1755982353271955No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT17520.1492712801034675No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC17150.1461188615168075No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT16170.13776921228727565No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA14120.12030310930713248No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA14120.12030310930713248No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG13880.11825829725092059No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG13660.11638388619939304No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC13540.11536148017128708No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA12920.11007904902607306No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT12670.10794903646751902No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC12090.10300740733167363No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA11860.1010477957778039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33650.062.9140321
GTATCAA60500.054.6514931
TATCAAC77250.042.647532
ATCAACG81300.040.376733
CAACGCA83400.039.3600465
AACGCAG86100.038.1948286
TCAACGC86700.037.9305044
ACGCAGA99700.032.927867
CGCAGAG99950.032.7873958
GCAGAGT101600.032.313469
GCTATTG7600.031.3028968
ACTCCTA12500.030.9259437
TCCTACG12550.030.8040479
TTTAGGG8650.028.8745842
ATGGGTG7350.028.3180485
GGGCTAT8400.028.3180476
CTAGAAC850.007229448827.9848963
CTATTGA8300.027.946329
CATGGGT19000.027.8560184
GGCTATT8600.027.6618447
Produced by FastQC (version 0.11.5)