FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2845072
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC146390.5145388236220384No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT76610.26927262297755555No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT76370.2684290590888385No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA57350.20157662090801218No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT57250.20122513595438007No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC53600.18839593514680825No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG53280.1872711832951855No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC52330.1839320762356805No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT50700.17820287149147718No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG42460.14924051131219174No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC38630.13577863758808212No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG38270.13451329175500654No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT36340.12772963214990693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35510.12481230703476046No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG34730.12207072439643003No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA33980.11943458724418925No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG33180.11662270761513241No Hit
CTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCC31820.11184251224573577No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA31350.11019053296366489No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT30470.10709746537170235No Hit
GTTCCGACCTGCACGAATGGCGTAATGATGGCCAGGCTGTCTCCACCCGA30210.10618360449225889No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC30130.10590241652935321No Hit
CCTTTACTATAGCTTGACACTGAACATTGAGCCTTGATGTGTAGGATAGG29740.10453162521018801No Hit
ATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATC29320.10305538840493315No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG28820.10129796363677263No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT28630.10063014222487163No Hit
GTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGGTTAGCGCAAG28470.10006776629906027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA186450.087.719661
GGTATCA91850.070.594541
TATCAAC278050.059.293222
ATCAACG301700.054.7883953
TCAACGC311700.052.938324
AACGCAG333950.049.368116
CAACGCA338250.048.8894425
CGCAGAG339500.048.4338848
ACGCAGA340250.048.411727
GCAGAGT341200.048.0221569
ACTGTGC81300.038.5921368
CTGTGCA79400.038.426699
CACTGTG80000.038.0498777
ACACAGC89150.033.4374581
CTATTGA14850.033.4356889
AGCACTG93300.033.2428675
TTCTCTG8400.032.554217
GCACTGT95750.032.0916446
TCTATAC30050.030.6526743
GGTCTAT32750.030.3404521
Produced by FastQC (version 0.11.5)