FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5002654
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227870.45549822154400443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122990.24584950308376313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115920.23171700461395092No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA51730.10340511256624985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA228250.064.234781
GGTATCA109700.062.4105031
TATCAAC325500.046.591042
CAACGCA335300.045.2997365
ATCAACG338350.044.874283
TCAACGC339900.044.687134
AACGCAG343950.044.1591726
ACGCAGA370300.041.032517
CGCAGAG377300.040.2857788
GCAGAGT402150.037.8407339
AGAGTAC392900.031.09418110-11
CAGAGTA397100.028.25141110-11
AGTACGG291450.027.5747312-13
GAGTACG294350.024.42639512-13
GAGTACT168300.023.90245812-13
TACGGGG95950.023.83129514-15
AGTACTT175550.023.69382912-13
GTACGGG297100.023.53970114-15
TACGGGC84700.023.4508914-15
TACGGGT56850.023.01380714-15
Produced by FastQC (version 0.11.5)