FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4081223
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211860.5191091003848601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90050.22064464499979541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89940.2203751179487129No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG61800.15142519778997618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA166850.068.354731
GGTATCA75900.057.0640141
ATCAACG221350.051.6789783
TCAACGC224700.050.9614524
CAACGCA226100.050.6979065
AACGCAG234150.049.0565456
CGCAGAG249100.046.0401548
TATCAAC254450.044.932972
GCAGAGT264750.043.453439
ACGCAGA267250.042.9801647
ACGGGAC25600.032.2989923
GTACGGG254150.031.5787221
CGGGCTA7950.031.4265214
TACGGGG94800.029.742962
AGAGTAC300850.029.56922310-11
CAGAGTA275950.028.62669610-11
ACGGGGA27150.028.0449263
TACGGGA105650.027.6456092
GAGTACG195000.026.9705831
ACGGGCT18850.026.5083143
Produced by FastQC (version 0.11.5)