FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4081223
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG59880.1467207256256274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115550.065.3748251
ATCAACG145000.051.8917053
TCAACGC146900.051.2610634
CAACGCA146700.050.960125
AACGCAG153200.048.906136
CGCAGAG159850.046.6819888
GCAGAGT168950.043.991499
GGTATCA54950.043.0072481
ACGCAGA180450.041.5207527
TATCAAC183900.041.0122642
GTACGGG275750.034.1946751
ACGGGAC29050.034.015813
TACGGGA117600.031.3330152
TACGGGG100000.031.0139142
TCTGTCG4250.030.8033668
CGGGACT12450.029.1661644
ACGGGGA29450.028.096253
ACGGGTA11450.028.0741833
GAGTACG205900.027.6099661
TACGGGT41150.027.4854352
Produced by FastQC (version 0.11.5)