FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004916480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205283
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77070.6394348879059939No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT35790.2969427097204557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34840.2890607434104687No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA33230.2757028847167014No Hit
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG32730.27155448139565563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32000.26549781254692884No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.2148043239637496No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT20160.16726362190456515No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA16520.13706324572735198No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16490.13681434152808927No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT16070.1333296827384108No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14090.11690200558706959No Hit
ATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTG13960.1158234207235977No Hit
GGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCC13950.11574045265717678No Hit
CTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC13390.11109424093760552No Hit
CTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGC13060.10835629474571533No Hit
GAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTT12320.10221665783056759No Hit
GCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGACCCTCGCCATCCTT12280.10188478556488394No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)