FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4542207
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86020.18937930393749117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65150.067.774621
GTATCAA150800.064.993491
TATCAAC202050.048.15342
TCAACGC203600.047.4068534
ATCAACG206300.046.901793
CAACGCA207000.046.456225
AACGCAG208550.046.3677526
ACGCAGA216550.044.488937
CGCAGAG226700.042.4707878
GCAGAGT242500.039.6295669
AGAGTAC222450.033.74456410-11
CAGAGTA230200.030.15285110-11
AGTACGG246700.029.207412-13
GAGTACG253700.026.41974412-13
TACGGGT46750.024.88145814-15
GTACGGG267950.024.58128414-15
TACGGGA96900.024.22333314-15
TACGGGG96900.023.21019214-15
TACGGGC63500.022.20676614-15
ACGGGAT24050.018.18352916-17
Produced by FastQC (version 0.11.5)