FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866052
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92661.0699126611335117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50800.58656985954654No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31220.3604864373039956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27850.3215742241805342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.25298711855639155No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT17500.2020663886233159No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC17190.19848692688198863No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15810.18255254880769284No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT14410.16638723771782757No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG13070.15091472567467082No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA12170.14052273997404313No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT11720.1353267471237293No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA11340.1309390198279087No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT9930.1146582422302587No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC9130.10542092160747854No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTGCAAAGTAAG8780.1013795938350122No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7400.081.999191
GTATCAA15800.071.322991
ATCAACG21100.052.884613
TCAACGC21200.052.635164
CAACGCA22450.050.0280275
AACGCAG22600.049.3774226
TACGGGT2800.046.280134
GTACTTT36150.045.7603341
ACGCAGA25300.044.674457
CGCAGAG25600.044.4321378
GCAGAGT26200.043.689389
GTACGGA1002.8682498E-643.1972776
ACGGGTC855.5655277E-542.3502735
ACTTTTT42150.039.6251073
TATCAAC30500.036.8239142
TACTTTT48050.034.461932
GAGTACG13300.033.131431
GTCTCGC1551.5068053E-632.6233331
AGTACGG14100.032.1681862
GTACGGG18350.031.4931371
Produced by FastQC (version 0.11.5)