FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004916521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66327304
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTTGATGATGATTCCATTCAATGATTCCATTCGATTCTATTCGAAAA20217913.0482031954743705No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3898480.5877639772604055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400590.36193088746679647No Hit
GCACCCTTTTTCCTGATATACTGAGGACACTCGGTCTCTAGCAATTTCTT2327620.3509293849784698No Hit
CCTCTGGACTCCATTTGATGATGATTCCATTCAATGATTCCATTCGATTC1301120.1961665741758477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1203360.1814275460374509No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1108990.16719961963175828No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT865130.13043346372106426No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT806700.1216241202868731No Hit
CCCTCGCCATCCTTGCTGCCATTCTCCTGGTGGCCCTGCAGGCCCAGGCT738360.11132067119749055No Hit
GATTCTATTCGAAAATGATTCCATTTGAATCCATTTGATGATGATTCCAT729450.10997733301507326No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)