FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068078
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA18690.17498722003449185No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC16240.1520488204045023No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC15340.14362246952001634No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC14170.13266821337018458No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT12770.11956055643876197No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT11700.10954256149831754No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG11570.10832542192611401No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC11530.10795091744235907No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA11340.10617202114452315No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG11230.10514213381419708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32300.052.4698221
GTATCAA50900.049.0680471
TATCAAC63200.039.424322
ATCAACG65950.037.1492233
CAACGCA67400.036.1735575
AACGCAG68700.035.6621676
TCAACGC70300.034.93514
CGTTAAC1152.7728905E-531.0285429
CGCAGAG79850.030.6079448
ACGCAGA79800.030.5526057
ATACGCG800.005377126429.7328991
GCAGAGT82600.029.6636859
TATACCG2052.4410838E-929.007715
GTAGTGT2205.185939E-927.029916
TACATGG109400.026.036672
GTACATG109900.025.8641071
CATGGGT18050.025.6971344
GTACTGG9150.025.3460771
ACATGGG105750.025.1358993
GGTCCGC4300.024.894999
Produced by FastQC (version 0.11.5)