FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004916545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3898975
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225890.5793573952128445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111130.28502362800479614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107880.27668810392474946No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA71290.1828429266666239No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC57970.1486801018216326No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAAAAAAAAAAAAAAAAAAAAAAA53670.13765156226957084No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC52830.1354971498919588No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52480.13459947806795375No Hit
GTACAGGAATCTCTACCTGTTATCCATCGACTACGCCTCTCGGCCTCGCC49900.1279823543367167No Hit
TTCCTGTACTGTGTCAAATCGTTAAGAGCGAAGGAGGGACGCAGGAGGTG47280.12126263953987906No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT46520.11931340929346816No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT43840.11243980789822966No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)