FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4433351
Sequences flagged as poor quality0
Sequence length150
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT312660.7052453099247048No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA202290.45629141477857266No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAG160100.3611263804738222No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152150.34319412110613395No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT146230.32984079086000634No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATAT130300.2939086032213556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115970.2615854237573339No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG98750.22274347327788843No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT83970.18940526026475232No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA81500.18383385389516868No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC70190.1583226773607594No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT64010.14438288328625457No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT63210.14257837919894004No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT61840.1394881659494139No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC60840.1372325358402707No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC59450.1340972099885617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56890.12832279690915516No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT54650.12327018546467446No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG53470.12060854193588551No Hit
TTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTA53110.11979651509659396No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA53000.11954839578458823No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC52890.11930027647258248No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48760.10998452412182118No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAAT48010.10829280153996379No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT47980.1082251326366895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45990.10373642871949458No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG45570.10278906407365444No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG44620.10064621546996844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30750.059.4566651
GTATCAA76100.057.1658861
ATCAACG78250.054.3774223
TCAACGC79300.054.292954
CAACGCA82650.053.3142935
AACGCAG83800.052.410816
CGCAGAG87950.050.097538
ACGCAGA91150.048.338227
GCAGAGT98350.043.3359269
ACGGGAC12950.041.1412433
GGGTTAG136450.040.7851267
GGGGTTA139400.040.1838846
AGGGGTT134950.039.0013435
ATAGGGG141150.038.45973
ACCTTAG89800.038.144081
GTACATG37450.038.013651
TAGGGGT143850.037.387484
TACATGC37200.037.3588522
CCTTAGC93350.037.025942
CATGCTA38450.036.8880234
Produced by FastQC (version 0.11.5)