FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052768
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246792.3442011915255785No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96310.9148264384935711No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66100.6278686282257819No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49560.4707589896349433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43840.41642603118635824No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26440.25114745129031274No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG21240.20175385270069002No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT18710.17772196723304662No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT16000.15198030335268548No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG14310.1359273838110581No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA13100.12443387337001124No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA12860.12215416881972095No Hit
GCACCCTTTTTCCTGATATACTGAGGACACTCGGTCTCTAGCAATTTCTT11220.10657618772607069No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.078.077671
GTATCAA33100.071.787921
GTCTCGC3950.064.585161
GTACTTT82300.063.8553431
TCTGTCG3700.062.260668
CGTCAAT1650.061.0814826
TCTCGCT4600.059.4747282
ATCAACG40500.057.9521183
TCAACGC40850.057.4528544
CAACGCA41500.056.897245
ACTTTTT97200.053.9967963
AACGCAG43850.053.8480156
TACTTTT99450.052.7751462
CTCGCTC5050.052.7493483
CGGGACA2050.052.6772964
ACGGGAC3150.052.568313
CGCTCTG4750.051.528895
ACGCAGA47150.050.2319077
CGCAGAG47600.049.7570278
GTCGTGC1056.622031E-848.5926441
Produced by FastQC (version 0.11.5)