FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2996400
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132400.4418635696168736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89730.2994593512214658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66230.22103190495260983No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA35010.11684020824989988No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT31020.10352422907488987No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC30250.10095447870778268No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52150.082.988541
GTATCAA122050.065.895471
ATCAACG168300.048.4587673
TATCAAC171650.047.5863082
CAACGCA174500.046.736245
TCAACGC174800.046.6900674
AACGCAG178150.046.012436
ACGCAGA188850.043.2479327
CGCAGAG189450.043.079568
GCAGAGT202800.040.185049
GACCGTT4450.037.429147
AGAGTAC198950.032.8000510-11
CAGAGTA204500.030.47000110-11
GGCGTCG2451.8189894E-1129.1358018
AGTACGG137850.027.06559212-13
GAGTACT98950.026.55043612-13
GAGTACG137100.026.19368212-13
GGACCGT14000.024.2191336
TACGGGT23050.023.88760214-15
ACGGTAC8300.023.6512933
Produced by FastQC (version 0.11.5)