FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793739
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125540.4493619482707583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72100.2580770787822341No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59720.21376370519937618No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43450.1555263394325669No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA36730.13147255344898No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC33330.11930248315966524No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC31020.11103399422780726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA192850.069.960131
GGTATCA123300.056.8974041
TATCAAC280650.048.304232
CAACGCA305000.045.1087465
ATCAACG305700.044.7707443
TCAACGC312800.043.9148644
AACGCAG335550.040.9160166
ACGCAGA342500.040.0857477
CGCAGAG342400.040.0119178
GCAGAGT348350.039.3284959
TATTCTC14600.030.5730485
ATTCTCT15100.030.037486
TATGGTG13600.029.6448354
CTATTGA17550.029.5351859
TCTCTGC14350.029.097828
GTGGGTA19050.025.6979378
ATTTAGG20300.025.479541
TTTAGGG20000.025.1981092
TAGGCAT11150.025.1819655
GTCTATA9900.025.0191761
Produced by FastQC (version 0.11.5)