FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7953961
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTATACTTCTCCAATACTTGTCTTAGCTTGTTTTTCATTTTCTATCT206140.2591664706427401No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA194310.24429337785287103No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC175040.22006645493987212No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA168460.21179384711592125No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC124160.15609832635588736No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC123820.15567086637714214No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC123010.15465250583954335No Hit
ATTTAAACCTGAAAAGTAGGAAGCAGAAGAAAAAAGACAAGCTAGGAAAC107150.13471275506631222No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC106260.13359381571018517No Hit
CTCCAATACTTGTCTTAGCTTGTTTTTCATTTTCTATCTGAAACTCTTGA99970.1256858061033993No Hit
GTCTTAGCTTGTTTTTCATTTTCTATCTGAAACTCTTGACAATATTTTCA90230.11344033494758146No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA86430.10866284106748826No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC84450.10617351530891339No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG83940.1055323253407956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69900.060.07681
GTATCAA146650.059.5419771
ATCAACG177700.048.021063
CAACGCA181650.047.0080955
TCAACGC184300.046.4949654
CGCAGAG195150.043.827488
TATCAAC202650.042.7847632
AACGCAG202050.042.743956
ACGCAGA207300.041.4607247
TCTATAC82050.041.455743
GCAGAGT213150.040.0148169
ACTTCTC86250.036.385568
AGAGTAC208300.033.05245210-11
TTCTATA116000.029.9899732
CAGAGTA221900.028.46904410-11
CTCTGCA63750.026.664919
AGTACGG257300.025.9611212-13
TCTGTCG8500.025.872428
TACTTCT130350.025.626737
TCTCTGC70250.025.3821958
Produced by FastQC (version 0.11.5)