FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987177
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC15540.1574185784312236No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA14790.14982115669226492No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC11930.12084965512770252No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT11590.11740549060604126No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC11370.11517691356261339No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT10750.10889637825840755No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG10300.10433792521503235No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA10110.10241324504116282No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAC300.004243501559.474751
GGTATCA23050.058.0556141
GTATCAA38000.053.370761
ATCAACG50800.039.690873
CAACGCA52050.038.7376825
AACGCAG53750.038.0657736
TATCAAC53350.038.014792
TCAACGC55200.036.6348534
GTGATCG3150.033.9872938
CGCAGAG61650.032.7055368
ACGCAGA61700.032.679037
GCAGAGT63400.031.898219
TGATCGC3400.031.4898229
ACAGTCC1254.8943766E-528.5493268
ATAAGGT4050.027.9031493
CGTTAAC1505.3067033E-627.7576969
GTACTGG9700.027.591381
TCTAGAG2205.173206E-927.0353453
GGTTCAC7300.026.8872096
GGTATGA1150.001007203725.8585851
Produced by FastQC (version 0.11.5)