FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889893
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC106190.5618836621967488No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT53350.28229111383554517No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT42760.2262561954565682No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG38890.2057788456806814No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT34770.18397866969188204No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC34330.18165049555715587No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT30890.16344840686747875No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA29120.15408279728005764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29000.1534478406978596No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA27730.1467278835362637No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG27660.14635749219664818No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG27290.14439970940153754No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC26940.14254775270345993No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT26030.13773266528845812No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC21510.11381596735899863No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA20940.11079992359355793No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC20200.10688435800333669No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC19710.10429161862602802No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT19580.1036037489953135No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG19380.10254548802498342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA136500.075.633951
GGTATCA78750.055.790661
TATCAAC197150.052.5259132
ATCAACG214800.048.1428833
TCAACGC221300.046.7288364
AACGCAG234050.044.5214736
CAACGCA235300.044.284965
ACGCAGA238200.043.534337
CGCAGAG238150.043.392388
GCAGAGT239550.042.950589
TTTAGGG13100.041.2002262
CTATTGA13950.040.7457289
CTGTGCA52000.040.1258139
ACTGTGC55200.039.7621968
CACTGTG54000.038.7800947
GCTATTG14400.037.9805038
GGGCTAT15950.036.0943266
GGCTATT15700.035.7523467
ACACAGC60500.033.1943241
ATTTAGG17000.032.8383451
Produced by FastQC (version 0.11.5)