FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586048
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227430.8794500334100527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171570.6634447620461801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125120.48382705966787937No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44940.17377867696191254No Hit
TTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34250.13244147053728314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG250.00206003571.374694
GGTATCA61500.063.3462261
GTATCAA130400.062.6705781
TATCAAC198550.044.964052
ATCAACG199550.044.7097783
TCAACGC200050.044.5385634
CAACGCA200700.044.3646855
AACGCAG204200.043.6042676
ACGCAGA231500.038.5135577
CGCAGAG236500.037.6993148
TAGGTCG801.2003875E-437.1743135
GCAGAGT253350.035.2154589
TTATCAA24500.032.045161
ATACGAC2850.031.3046883
AGAGTAC248450.030.75032210-11
AGTACTT151300.029.817512-13
GTATACG1254.8967602E-528.5498773
GAGTACT142600.027.4657112-13
TACATGG164350.026.2013342
ACATGGG157900.026.0667513
Produced by FastQC (version 0.11.5)