FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318699
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100780.43464028750605405No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC53080.2289214770869354No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC48340.2084789789446582No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA47610.20533066172021466No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT44550.19213360595747875No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC42890.18497441884436058No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT42470.18316305824947524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.182990547716629No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40150.17315744734439442No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC35170.1516798860050399No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA34640.14939412144482747No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG31390.135377640651072No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT29370.12666585874233782No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC28040.12092988352520098No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC27700.1194635439960081No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT26910.11605646097229524No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC26900.11601333333908369No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT26810.11562518464017968No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC25660.11066550682085083No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT24570.10596459480079129No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC23380.10083240644861621No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104650.075.9063341
TACAGAG20050.072.675126
GGTATCA44100.067.969881
CAGAGAA21100.067.649228
AATACAG23350.065.205934
AAAGGTC9400.060.107657
ATACAGA24650.059.5955775
CATGTAA4650.057.5563962
ACAGAGA26900.054.3897257
AGAGAAA28000.050.978529
ATCAACG158550.050.715943
TATCAAC159850.050.4151042
CAACGCA165700.048.4557465
TCAACGC171250.046.9895484
AACGCAG173600.046.4562346
TCATGTA6000.045.6871071
ACGCAGA176350.045.529447
CGCAGAG178050.045.1281368
TAAAAAG12850.044.4326444
GCAGAGT181050.044.413219
Produced by FastQC (version 0.11.5)