FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318699
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC65260.2814509343386097No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA52050.2244793308661452No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC50810.21913150434791237No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC46000.19838711277315427No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT45300.1953681784483454No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT39960.17233802231337486No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA36510.1574589888553883No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT32500.14016480793755465No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG32010.13805155391018845No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC30570.13184117472772447No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT28470.12278437175329787No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC28250.12183556382264366No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC27850.12011045849418144No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC27760.11972230979527744No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG25280.10902665675881172No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT24820.10704278563108019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86550.074.921791
GGTATCA37400.066.012351
ATCAACG130100.049.3392873
TATCAAC133950.048.4541242
CAACGCA138650.046.2538225
TCAACGC141150.045.603184
AACGCAG145400.044.3111276
ACGCAGA144500.044.134247
CGCAGAG146000.043.8030478
GCAGAGT147850.043.2147189
AGAGTAC149350.032.4838510-11
TCTCTGC12500.030.9351378
CAGAGTA147650.030.15831410-11
ACTGTGC31050.028.547858
CACTGTG29500.027.4261997
GGTCTAT16200.027.1747421
AGTACGG177850.026.9606212-13
GTCTATA16250.026.725032
TATAAGA6800.026.2458762
TCTATAC18300.025.0315863
Produced by FastQC (version 0.11.5)