FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13534686
Sequences flagged as poor quality0
Sequence length150
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1575881.164326974412262No Hit
ACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA886630.6550798444825392No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT781500.5774053420965953No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG617770.45643467458351084No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT528520.3904929896415772No Hit
CCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAG485650.35881881559719964No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT419780.3101512661616236No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT331160.24467505193692707No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA316800.2340652749535527No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA297090.21950269108570378No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG269390.19903675637543422No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC261780.1934141656481724No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA252170.18631389010428465No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA235360.17389394922054344No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT232880.17206162004792722No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT230810.17053221626271936No Hit
TCTTTATCACCAGAATGAGGAACTCCTGGAAGTTAACTGCACCATCAGTG220980.16326939538900276No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT218550.1614740083368022No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT217380.160609562719076No Hit
TCTGAGATGTTAGTGTTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC208650.15415946849450368No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC204580.15115237989266983No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT203410.15028793427494366No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT202610.14969686034829327No Hit
CTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGAT202170.14937176968863555No Hit
GTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATGTTGT200960.14847777037457685No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCAT200600.14821178710758418No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT196500.14518253323350094No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA188550.13930873608741273No Hit
GTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGT187480.13851817471051786No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG187390.13845167889376966No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC183490.13557019350134905No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG180840.13361226111931965No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT178930.13220107211944188No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT174040.12858813274279138No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT173150.12793056299939282No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG166920.12332757479560294No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG159840.11809657054474704No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA157180.1161312497386345No Hit
GGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCG154310.11401077202677623No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC152350.1125626409064828No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT150560.11124011299560256No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT146950.1085728919015927No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA146520.10825518966601812No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA142540.10531459688093243No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC136360.10074855079755822No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGC135810.10034218747298608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGC144250.093.631052
GTACATG147550.093.065911
CATGCTA171150.079.379644
TAAGACT177500.076.05489
ACATGCT180000.075.076553
TGCTAAG212150.063.362856
GGTATCA51550.060.9548531
CGGGACA22450.059.650044
CCTGGGG26050.057.7631763
GGGGTTA463100.056.5770956
CCCTGGG27800.055.4206852
CAACGCA173150.054.0568855
GGGTTAG484200.053.8873867
ACGGGAC37350.053.2023743
ATCAACG175350.053.0069733
TCAACGC175900.052.964224
AACGCAG175500.052.7180566
CGCAGAG175550.052.3327568
CCGCAGT39900.051.958511
AGGGGTT476500.051.6765375
Produced by FastQC (version 0.11.5)