FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6337644
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT447240.7056881074418191No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG385420.6081439727444458No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT359690.5675452897007153No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT339130.5351042122277616No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA278510.4394535256319225No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT125260.19764442433181795No Hit
GGATAACACAAATATCAGAAGTTCTTTCACAGTTAGATTGAAACTGGAAA124830.1969659387621015No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA119410.18841386483683842No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT119250.18816140508996718No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT116010.18304909521582466No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG111490.17591710736671232No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT102960.1624578471116396No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT102200.16125866331400124No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA99900.15762955445272725No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG97010.15306950027486554No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT96520.15229634230007238No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC91470.14432808153944907No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCAT88440.13954712508307504No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT86000.1356971139432887No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG83620.13194177520857908No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG82320.1298905397652503No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG81570.12870713470179138No Hit
ATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACC81290.12826533014476674No Hit
CATAAGGATAACACAAATATCAGAAGTTCTTTCACAGTTAGATTGAAACT71780.11325975394010772No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC71510.1128337281172625No Hit
CAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATA70500.11124007596513784No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC67140.10593842128084191No Hit
AAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTA66320.10464456507812683No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA65320.1030666916601816No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATCT64680.10205685267269667No Hit
AAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCA64530.10182017166000489No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT63630.10040008558385419No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49400.050.0872571
GTATCAA109900.042.5929681
ATCAACG108550.041.809283
TCAACGC110300.041.307724
CAACGCA112000.040.893165
CCTTAGC95600.037.2674642
ATAGGGG166050.037.2181363
AACGCAG123900.037.1581846
ACCTTAG98350.036.6487461
TAGGGGT170200.036.380544
GGGGTTA180000.036.217876
AGGGGTT172750.035.636935
ACATGGG186650.034.5763973
CCCGTTG5350.034.4656034
CGCAGAG133900.034.1168488
GTACATG204550.034.079171
TAGCCAA106600.033.8695535
GGGTTAG194750.033.7194067
TACATGG199100.033.398842
TCTATAC32400.033.228563
Produced by FastQC (version 0.11.5)