FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6337644
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT501880.7919031109983458No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG331010.5222918800740465No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT321090.5066393757680299No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT290530.458419564115624No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA226910.3580352572659493No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA126240.19919074028140427No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT116600.18398004053241235No Hit
GGATAACACAAATATCAGAAGTTCTTTCACAGTTAGATTGAAACTGGAAA111320.1756488688856616No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT110090.173708084581589No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG106940.16873778331506156No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC105780.1669074501502451No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA105190.16597650483365742No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT101350.15991747090874778No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG98660.15567299141447516No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT97720.15418979040160666No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT97600.15400044559145323No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT94780.14955084255284773No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT93210.14707358128667372No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG80650.1272554911572818No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCAT75480.11909788558650501No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG69580.10978843242062825No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG68230.10765830330640219No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT63400.10003717469772679No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG77700.044.703863
TCAACGC79850.043.7932934
CAACGCA80550.043.55595
GTACATG220450.040.9040571
ACATGGG200700.040.8667533
GTATCAA93100.040.5696531
TACATGG211400.040.064362
AACGCAG89850.039.1762666
CGCAGAG92900.037.7543038
ATAGGGG182050.037.4736483
GGTATCA40450.037.0560651
TAGGGGT184600.036.7586144
CATGGGG69900.036.6695254
GGGGTTA200500.036.1203776
AGGGGTT189050.036.0154955
ACGCAGA98650.035.796747
GGGTTAG213050.034.2107937
CCTTAGC87150.033.236462
CATGGGT41150.032.517554
CATGGGA101950.032.4917534
Produced by FastQC (version 0.11.5)