FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2545822
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC146690.5761989644209219No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT106890.41986438957633326No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT71040.27904543208441124No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG68610.2695003814092266No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT67480.26506173644504605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59340.23308778068537392No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT56180.22067528680324075No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT53650.2107374356887481No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC50880.19985686351991616No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA48730.19141165407479394No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA48660.1911366937672783No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA44450.17459979527241104No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG41170.16171594086310828No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC39540.15531329370238767No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG39110.15362425181336323No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC39060.15342785159370922No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG38690.15197448996826957No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC36600.14376496078673215No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGAT36090.1417616785462613No Hit
TGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGAC35850.14081895749192205No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG34110.13398422984796265No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA33640.132138067783215No Hit
GTATAGACCCGAAACCCGGTGATCTAGCCATGGGCAGGTTGAAGGTTGGG31920.1253819002271172No Hit
AACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAA30690.12055045482362867No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC29910.1174866113970262No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT29660.11650461029875615No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC29020.11399068748718488No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG29010.11395140744325408No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA28960.11375500722360007No Hit
TTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCGTGTTTGC28800.11312652652070726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28780.11304796643284566No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG28630.11245876577388365No Hit
CGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTG28440.11171244493919844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28430.11167316489526763No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT28260.11100540414844401No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC27990.10994484296231236No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG26930.10578115830564745No Hit
GTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACT26860.10550619799813185No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT25940.10189243395649815No Hit
GTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTT25830.10146035347325932No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT25790.10130323329753611No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA159350.078.778621
GGTATCA90900.063.3892821
TATCAAC235950.052.996542
ATCAACG250650.049.8297163
TCAACGC253050.049.3571134
TACAGAG5400.048.7271846
AACGCAG260400.047.683336
CAACGCA266650.046.5390135
ACGCAGA267750.046.374387
CGCAGAG268900.046.1496058
GCAGAGT274800.045.210529
TTCTCTG10050.044.579777
ACTGTGC64700.043.526558
TCTCTGC10300.043.4977348
CACTGTG63150.043.243547
CTGTGCA63350.042.657989
TTTAGGG20450.041.0428352
ACACAGC68400.039.4184381
GCTATTG21650.039.0888068
CTATTGA21600.038.850059
Produced by FastQC (version 0.11.5)