FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4228135
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA140620.3325816228668195No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG121560.2875026459656563No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC117130.2770252132441372No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC102720.24294399303711922No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC89300.21120423070691924No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA82530.1951924430038303No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA76730.1814748109982297No Hit
TCATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTAC69580.16456428188787728No Hit
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTAC68780.162672194714691No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA64920.15354287410406717No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC64560.15269143487613332No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC63630.15049188353730428No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA62470.14774835713618417No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA52770.12480680016130044No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA52670.12457028926465213No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT49280.11655256986827527No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC46930.11099456379704055No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC44750.10583862625010791No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG43170.10210175408306499No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103700.060.3756371
AAAGGTC37500.056.4374057
GGTATCA48550.053.865791
AAGGTCA39700.051.8124288
AGGTCAA42850.047.3098839
CGTCGAC2900.047.149625
TACAGAG67100.046.8685466
ATCAACG145000.044.5256353
TCAACGC146150.044.093934
GAAATAC94200.043.1671642
CAACGCA152300.042.274345
ACAGAGA74950.041.95977
AACGCAG153650.041.941616
TATCAAC157050.041.2607272
CGCAGAG159350.040.3667268
ACGCAGA161650.039.8659327
ATACGGG4900.038.9037671
AATACAG84900.038.7927324
ATACAGA82500.038.6241725
TCATAAA87600.038.5581971
Produced by FastQC (version 0.11.5)