FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4193270
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC184660.44037231086956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166270.3965163225835684No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT146260.3487970009085988No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT140510.3350845521514236No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT109850.26196739060446855No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG95830.2285328633739301No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC94140.22450259582616908No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC92620.22087773980688105No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT90500.215822019569453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89710.21393804834890195No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA84760.20213341854924677No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA75710.18055121659230147No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC68920.16435860319035026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66430.15842051668506918No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC65990.15737121625843314No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG65770.15684656604511515No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC62860.14990687458713603No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT62390.14878603094959303No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA62180.14828522847324402No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG58530.1395808044795589No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT55350.1319972241234168No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG52680.12562987835269374No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC50390.12016874658679264No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA49940.11909559842318763No Hit
GTTCCGACCTGCACGAATGGCGTAATGATGGCCAGGCTGTCTCCACCCGA49940.11909559842318763No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT49870.11892866426440464No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA49840.11885712105349762No Hit
GCGTAATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACT49770.11869018689471462No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC48710.1161623267760006No Hit
ATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATC48300.11518456956027157No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG47420.11308596870699954No Hit
CTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCC46840.11170279996279753No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC45140.10764868467806749No Hit
CCTTTACTATAGCTTGACACTGAACATTGAGCCTTGATGTGTAGGATAGG44050.10504928134844643No Hit
GTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGT43570.10390458997393441No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43390.10347533070849241No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG43390.10347533070849241No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT43190.10299837596911242No Hit
GTATAGACCCGAAACCCGGTGATCTAGCCATGGGCAGGTTGAAGGTTGGG42770.1019967710164144No Hit
GTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACT42660.10173444590975539No Hit
CGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTG42540.10144827306612739No Hit
CTGATATGTAGGTGAAGCGACTTGCTCGTGGAGCTGAAATCAGTCGAAGA42400.10111440474856138No Hit
GTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTT42100.10039897263949137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA240900.087.4114151
GGTATCA129450.065.784561
TATCAAC360700.058.6506652
AACGCAG382800.055.096436
ATCAACG385200.054.789473
CGCAGAG387750.054.133278
CAACGCA390550.053.6911745
ACGCAGA390800.053.6739587
TCAACGC393350.053.525534
GCAGAGT395500.053.1452759
CTATTGA28250.043.302219
GCTATTG29600.041.5703858
ATACGGG11050.041.039093
TTTAGGG33500.037.3872832
TTCTCTG13450.035.845297
GGGCTAT35650.035.7267386
CACTGTG104700.034.3639377
ACTGTGC113250.034.1840638
GGCTATT38000.033.896057
CTGTGCA109550.032.711219
Produced by FastQC (version 0.11.5)