FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987450
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29460.14823014415456992No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC27570.13872047095524415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93750.081.748631
GGTATCA46050.078.8832551
ATCAACG147850.052.665343
CAACGCA150400.051.7245675
AACGCAG150900.051.5531776
TATCAAC153350.051.011112
TCAACGC154550.050.149394
ACGCAGA155850.050.100487
GCAGAGT156300.049.5331349
CGCAGAG159550.048.8033268
CAGAGTA154300.029.2377599
ACTGTGC21250.027.4312158
CACTGTG20400.026.4575777
GTATAGA12400.025.72811
AGTACGG179800.021.8535462
GAGTACG181550.021.8058551
ACAGCAC23700.021.2553713
AGAGTAC154100.021.18192110-14
ACACAGC24200.020.673331
GCACTGT29350.020.5963466
Produced by FastQC (version 0.11.5)