FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004916760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496678
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATG91720.20397279947552394No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA75480.16785724928491655No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC70570.1569380773984706No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT57200.12720501668120332No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT54430.12104491360066254No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC52790.1173977767587539No Hit
TCATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATG48270.10734591180422526No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG46730.10392116135511592No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC45200.10051864954528655No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)