FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5680462
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC71320.12555316803457184No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA211100.073.201511
GGTATCA90300.059.700331
TATCAAC319150.048.322522
AACGCAG319650.048.0882156
TCAACGC322550.047.5708354
ATCAACG322400.047.5384453
ACGCAGA322150.047.5132457
CAACGCA322900.047.476975
CGCAGAG330950.046.2670148
GCAGAGT349250.043.743649
AGAGTAC337800.036.16108710-11
AGTACGG393750.030.79553812-13
CAGAGTA346500.029.72660410-11
TACGGGC104400.026.71963114-15
TACGGGT91950.026.68273514-15
GAGTACG394550.025.93999712-13
TACGGGA135550.025.07695814-15
GTACGGG420100.023.48123614-15
TACGGGG136100.023.03088614-15
ACGGGTA25250.020.38020916-17
Produced by FastQC (version 0.11.5)