FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4097893
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323570.789600899779472No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193210.4714862003473492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162780.39722852695275357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128480.3135269759361701No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88160.215134948618717No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82880.20225027837476478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107300.075.130211
GGTATCA48050.069.690141
CAACGCA130200.060.323065
TCAACGC130950.060.0391274
ATCAACG131100.060.0268173
AACGCAG133850.058.8401576
ACGCAGA145950.053.9620067
CGCAGAG146350.053.863718
TATCAAC152050.052.1822742
GCAGAGT170400.046.388219
TCTGTCG10950.046.0212948
GTCTCGC10050.042.829011
CTCGCTC10550.039.5825163
TCTCGCT13200.034.363272
CGCTCTG12400.034.25315
GAGTACG69950.032.435811
AGTACGG71950.031.5215532
TCGCTCT15200.030.3147284
CTGTCGC16450.029.3213379
GTACGGG88500.027.5331081
Produced by FastQC (version 0.11.5)