FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4097893
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151880.37062949179004917No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91470.22321227030574006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76660.18707174638283625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56110.13692402412654503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76500.068.952331
ATCAACG86600.060.6905333
TCAACGC87100.060.094154
CAACGCA89750.059.3662725
AACGCAG90350.058.3344886
ACGCAGA96100.054.6153647
CGCAGAG95550.054.4789738
GGTATCA36350.052.4791831
TATCAAC111900.046.6510052
GCAGAGT112950.045.385289
GTCTCGC10700.042.5445861
TCTGTCG13450.038.5417568
CTCGCTC11000.035.344113
GAGTACG68350.032.6668661
AGTACGG69800.032.2880632
CGCTCTG12400.031.9362185
TACGGGT13300.030.3146364
TCTCGCT15500.027.407752
TCGCTCT15100.027.1777744
GTACGGG85500.026.5369531
Produced by FastQC (version 0.11.5)