FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4820555
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA89700.18607815905015088No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC73240.1519327131419515No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC71610.14855135975007028No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG71020.1473274342892053No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT70990.1472652007911952No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA63490.13170682628867422No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT63170.1310430023099No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA63150.13100151331122661No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC62820.1303169448331157No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT62570.12979833234969831No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG60460.12542124298965576No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT56420.11704046525763112No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC55880.11592026229344961No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA54300.11264263139825187No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT53860.11172987342743729No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAG51650.10714533907402778No Hit
GTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTT51350.10652300409392694No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG50860.10550652362642891No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG49630.10295495020801546No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGA48270.100133698298225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69050.084.9411541
GTATCAA160500.063.2649881
ATCAACG213900.047.136723
CAACGCA220200.045.7881245
AACGCAG221750.045.5466466
TCAACGC221600.045.5256924
ACGCAGA223350.045.060587
CGCAGAG225000.044.8604558
TATCAAC225750.044.7419472
GCAGAGT231600.043.6590969
CAGAGTA239500.032.9932110-11
AGAGTAC241400.032.42599510-11
CTCTGCA14250.030.0525939
ACTGTGC19650.029.3611818
GTACTGG26300.029.1768861
TTCTCTG15850.028.1458517
ATTTAGG30300.026.6993851
GAGTACG296450.026.34447312-13
ACGGGTT21050.025.9977745
GCTCTAG4150.025.8005731
Produced by FastQC (version 0.11.5)