FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4048012
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT114030.28169382897086276No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT60770.15012307275768944No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG57830.14286024843799872No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA51270.12665476288113772No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT46110.11390776509555801No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA246050.042.3396951
ATATACG20150.034.8411061
ATACGAC20200.034.7548643
ATCAACG298400.034.2139633
TCAACGC301250.033.8112834
TATCAAC308600.033.565092
CAACGCA303850.033.4828035
GACACTC21050.033.341567
GGGGTTA50900.033.0733646
AACGCAG310250.032.8439456
TATACGA21450.032.7295232
GGTATCA147800.031.2372111
GATCTAC28650.030.9418721
AGGGGTT53450.030.8330845
ACGCAGA340950.029.8305637
GCTCTAG2800.029.7478071
ATAGGGG58450.029.7223593
CGCAGAG342000.029.6809278
GGGTTAG59200.028.935227
CTACTGG13600.027.9837389
Produced by FastQC (version 0.11.5)