FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305702
Sequences flagged as poor quality0
Sequence length125
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194880.4526091215787809No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71470.16598919293532158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65810.15284383359554377No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC57990.13468187069146914No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA213250.068.220621
CTATCAA59400.054.3314251
TATCAAC343950.052.4632722
TCAACGC355450.051.1910554
ATCAACG351950.051.173883
AACGCAG361550.050.5447046
CAACGCA363250.050.2309045
ACGCAGA376150.048.523167
CGCAGAG385150.047.4913028
GGTATCA90700.047.277641
GCAGAGT397850.045.8765569
CGTATCA9050.045.2734871
CTACGGG18600.036.5125471
AGAGTAC392700.032.7505510-11
CAGTACG12250.030.2397061
CAGAGTA398850.029.14272110-11
AGTACGG329750.028.23717112-13
GAGTACT122150.026.90252312-13
AGTACTT134600.026.18630412-13
TACGGGC81350.025.90669314-15
Produced by FastQC (version 0.11.5)