FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305702
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC67600.15700111154928975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA201050.070.737361
TATCAAC288150.049.251532
GGTATCA94750.048.2949941
ATCAACG298450.047.4520873
TCAACGC298950.047.2333954
AACGCAG301450.046.7836
ACGCAGA306000.046.026897
CAACGCA306800.045.9865885
CGCAGAG313500.044.8688288
GCAGAGT326400.043.1492049
AGAGTAC320850.033.2037310-11
CAGAGTA324850.028.51808510-11
AGTACGG381750.027.40724212-13
TACGGGC100400.025.39315214-15
TACGGGT85500.024.07739614-15
GAGTACG384850.023.67704612-13
TACGGGA137850.022.48694414-15
GTACGGG392700.021.90063514-15
TACGGGG107850.020.96345914-15
ACTGTGC67200.018.683068
Produced by FastQC (version 0.11.5)