FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7489014
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301900.40312382911822575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177900.2375479602521774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116410.1554410233443281No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA80350.10729049244666922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA482600.046.946141
ATCAACG587850.038.1264573
TCAACGC593850.037.6911664
TATCAAC599600.037.587392
CAACGCA597000.037.4723635
GGTATCA293850.037.1033441
AACGCAG609850.036.7122966
TATACGA19350.035.350542
CGCAGAG674800.033.117458
ACGCAGA676350.033.0853087
GATCTAC30000.032.317861
ATACGAC21000.031.1569883
GCAGAGT734200.030.4946129
TACGACA21800.030.013614
CATGGGT108250.028.4632134
TCTACAC44950.027.788923
ACGACAC22700.026.9894145
GAGTACT232050.026.51749212-13
CTACACG17500.026.5117634
ACATGGG687050.025.9379433
Produced by FastQC (version 0.11.5)