FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7489014
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA360400.039.343791
ATCAACG423450.032.851393
TCAACGC424400.032.692684
CAACGCA430750.032.1546445
TATCAAC446500.031.5683982
AACGCAG437350.031.5551366
CGCAGAG484900.028.3183338
ACGCAGA490500.028.105417
TATACGA25350.027.9186672
GGTATCA236750.025.9516541
ATACGAC26400.025.6818683
GCAGAGT538300.025.5522679
TACGACA26000.024.9324114
GTACATG705600.024.183921
TACATGG695150.024.0752752
ACATGGG665650.023.9092433
CATGGGT109400.023.4842974
CATGGGG164500.023.2464284
GATCTAC41300.023.186251
ATATACG27800.022.8925271
Produced by FastQC (version 0.11.5)