FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004916804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA291.2048192771084338TruSeq Adapter, Index 25 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.7893643539675945No Hit
GAAGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA110.4570004154549232RNA PCR Primer, Index 26 (96% over 31bp)
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGA100.4154549231408392TruSeq Adapter, Index 25 (96% over 27bp)
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAC90.3739094308267553TruSeq Adapter, Index 25 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.3323639385126714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.3323639385126714No Hit
GAAGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAA60.24927295388450355TruSeq Adapter, Index 12 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT60.24927295388450355No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAG50.2077274615704196TruSeq Adapter, Index 25 (96% over 27bp)
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATC50.2077274615704196No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGC40.1661819692563357TruSeq Adapter, Index 25 (96% over 27bp)
GATCGGAAGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC40.1661819692563357RNA PCR Primer, Index 26 (100% over 26bp)
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGAA40.1661819692563357TruSeq Adapter, Index 25 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT40.1661819692563357No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA40.1661819692563357No Hit
TGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTG30.12463647694225177No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT30.12463647694225177No Hit
GATCGGAAGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC30.12463647694225177RNA PCR Primer, Index 17 (96% over 25bp)
GAAGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAA30.12463647694225177RNA PCR Primer, Index 27 (100% over 28bp)
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT30.12463647694225177No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGAAAAA30.12463647694225177TruSeq Adapter, Index 25 (96% over 27bp)
CTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCC30.12463647694225177No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC30.12463647694225177No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT30.12463647694225177No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT30.12463647694225177No Hit
ACTACAACCCTCGTAATAGGGCTGCGTGCGTCCTAAATACACTCGCTGTT30.12463647694225177No Hit
GTATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTA30.12463647694225177No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12463647694225177No Hit
CTGTTGAGATACTAAAATTATCTGTGGATTGCCGGCATTGAGCCCACGAG30.12463647694225177No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT30.12463647694225177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30.12463647694225177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)