FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4159568
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287040.690071661287903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152060.36556680886092013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82750.1989389282733207No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT79900.19208725521496461No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT75740.18208621664557473No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG71060.17083504825501108No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC67430.16210818046489445No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG67090.1612907878895116No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA57700.13871632823408583No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA56390.135566962723052No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA55940.13448511960857473No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA53290.12811426571220857No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA50840.12222423097783232No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT50760.12203190331303636No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG50370.12109430594715603No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC50180.12063752774326565No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT47750.11479557492508838No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA44150.1061408300092702No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA44040.10587637947017574No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT43320.10414543048701211No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC42060.10111626976647575No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC41620.10005846761009798No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84450.076.424281
GCGCAGA17500.074.7800751
GCTCTAG4650.072.916051
GTATCAA217150.068.83891
GCGTACT2550.058.317791
ATCAACG270400.055.6997263
TCAACGC280600.053.9505844
CAACGCA284300.053.2066045
GATCTAC4350.051.9631651
AACGCAG295150.051.290376
GCATAGA4850.050.285571
GCGTAAG1700.048.9869461
GTATACG1251.2732926E-1147.5873221
TATCAAC319500.046.767572
ACGCAGA335950.045.0795757
CGCAGAG349850.043.27158
GCAGAGT370500.041.116629
CGTACTT3350.040.8393752
ATACGAC3950.037.6477433
GTCTTAC4600.037.5008771
Produced by FastQC (version 0.11.5)