FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4159568
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG97010.23322133452320049No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT74240.1784800729306505No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT68740.16525754597592826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67410.16206009854869544No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG64220.15439103291495657No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC60870.14633731195162575No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA58860.14150507937362727No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA54440.1308789758936505No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA51720.12433983529058787No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA51120.12289737780461817No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG49360.11866616917910706No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA49120.11808918618471918No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG47480.11414646905640201No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA46260.11121347216826363No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT46200.11106922641966667No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT45890.1103239567185823No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT45740.1099633423470899No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT43410.10436179910990756No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC43110.10364057036692273No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC42740.1027510549172414No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA126500.064.432981
GGTATCA52650.060.7941931
ATCAACG168550.047.8281443
TCAACGC170000.047.4901924
CAACGCA171700.047.1591575
AACGCAG180550.044.7427836
ACGCAGA208400.038.5871057
CGCAGAG210800.038.0576368
TATCAAC221950.036.5085832
GCAGAGT223050.036.3940969
GCTCTAG3850.032.4516531
CATGGGT44600.029.3468024
TACATGG302100.028.141972
AGAGTAC224200.027.74475710-11
ACATGGG301150.027.5195433
GTACATG346400.027.463051
GTACACT11050.027.4554526
TCCAGTA12150.026.9314272
ATATACG5100.026.8309381
CATGGGG85100.026.4262314
Produced by FastQC (version 0.11.5)